Human cytomegalovirus (HCMV) replication and Ca2+respone in human cell lines of neuronal origin were investigated. SK-N-SH (neuroblastoma cells) and A172 cells (glioblastoma cells) were used. SK-N-SH cells were permissive for HCMV multiplication
with a
delay of one day compared to virus multiplication in human embryo lung (HEL) cells. The delay of HCMV multiplication in SK-N-SH cells appeared to be correlated with a delay in the Ca2+response. The cytoplasmic free Ca2+concentration([Ca2+])began
to
increase at 12 h p.i. in HCMV-infected SK-N-SH cells, while [Ca2+]i increase in HCMV-infected HEL cells was observed as early as 3 h p.i. On the whole, the level of the increase in [Ca2+]i in SK-N-SH cells was about 30% of that in HEL cells. On
the
other hand, in A172 cells infected with HCMV, neither production of in fectious virus nor detectable increase in [Ca2+]i was observed. Treatment with TPA of HCMV-in-fected SK-N-SH cells resulted in [Ca2+]i increase at 6 h. p.i. The stimulatory
effect of
TPA on HCMV-induced [Ca2+]i increase continued until 12 h p.i., but TPA failed to stimulate the Ca2+ response in SK-N-SH cells at 24 h p.i., suggesting that the effect of TPA had disappeared in SK-N-SH cells at that time point. In conclusion,
SK-N-SH
cells tre permissive for HCMV re-plication and the delay in Ca2+ response may be a consequence of the lower responsiveness of SK-N-SH cells than HEL cells to HCMV infection.
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